Urease and acetylcholinesterase enzyme inhibitor novel phthalonitrile azo compounds

Abstract

Based on the relationship between Helicobacter pylori and Alzehimer’s disease, new azo phthalonitrile compounds containing different amino pyridines have been synthesized. These compounds can inhibit both Urease and Acetylcholinesterase, which are the most important enzymes in the treatment of Helicobacter pylori and Alzehimer’s disease, respectively. All compounds have shown better inhibition than standards in the presence of both enzymes. Especialy, azo phthalonitrile compound 4 is more active than other compounds and standards, showed a significant inhibition performance and efficient IC₅₀ values to Urease (20.47±0.14 µM) and Acetylcholinesterase (4.73±0.07 µM) enzymes. Interestingly, the inhibitory effect of the newly synthesized compounds against to Urease and Acetylcholinesterase is same ranking.