Immunosuppressive and anti-cancer potential of aqueous extract of Solanum Xanthocarpum

Abstract

In this study whole plant aqueous extract of Solanum Xanthocarpum (HAESX) was investigated to assess its effect on humoral immune response along with interleukin-2 (IL-2) production and its expression in Wistar albino rats splenocytes culture. Anticancer potential of HAESX was investigated using rat lever hepatoma (N1S1 cancerous cell line). The effect of HAESX over humoral immune response was studied using four groups of five animals each (Group-I as control, Group -II orally fed with 125 mg/kg body weight, Group -III orally fed with 250 mg/kg body weight and Group -IV orally fed with 500 mg/kg body weight of HAESX). Quantification of IL-2 was done by sandwich ELISA and its expression was detected by the real time PCR. SRB assay (Sulforhodamine B) was done for detecting the effect of HAESX on N1S1 cell line. Dose dependent decrease in antibody titer was observed and production of IL-2 was also decreased significantly. Suppression of IL-2 production at 250 µg/mL and 500 µg/mL dose was also confirmed by the Real time PCR. Relative fold change in the expression of IL-2 gene was 592.22 and 10.77 at 250, 500 μg/mL HAESX concentrations respectively with respect to control. Dose dependent suppression of percent growth of N1S1 cells with increasing concentrations (10, 20, 40 and 80 µg/mL) of HAESX was found. It was concluded that S. xanthocarpum have the immunosuppressive, and anti cancer activity that can be further explore in treatment of various inflammatory and autoimmune disease.