Dynamic monitoring of the cytotoxic effects of Cousinia iconica extracts on A549 and RL95-2 cells using the xCELLigence RTCA system

Paşayeva, Leyla

Abstract

Asteraceae family plants have potential anticancer activity. The plants have various pharmacological effects and the effects of Cousinia iconica Hub. - Mor. (Asteraceae) depend on many factors, mainly on the type of cells and dose.  In this study, the cytotoxic effects of the C. iconica were investigated using different tumor cell lines: Non-small lung cancer (A549) and human endometrium carcinoma cell line (RL95-2). For the study, cell viability was determined with MTT assay and the xCELLigence Real‑Time Cell Analyser (RTCA) SP instrument was used. Different concentrations of C. iconica extract and sub-extracts were applied on A549 and RL95-2 cell lines.  The ethyl acetate sub-extract and methanol extract of C. iconica were cytotoxic to RL95-2 cells. According to RTCA results, the IC50 values of ethyl acetate sub-extract 44.82, 60.89, and 55.22 µg/mL and methanol extract 265.52, 250.48, and 246.65 µg/mL, for 24, 48, and 72-h exposure, respectively. Real-time xCELLigence measurements show that RL95-2 cells are more sensitive to ethyl acetate sub-extract than methanol extract. The results show that ethyl acetate sub-extract has a cytotoxic effect on RL95-2 endometrium cancer cells, with low IC50 values close to cisplatin. This study is the known first study to show the cytotoxic effects of C. iconica extracts on both A549 and RL95-2 cell lines.


Keyword(s)

Asteraceae; Cytotoxicity; LC-MS/MS; RL95-2; xCELLigence.

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