Antimicrobial and anti-inflammatory response by two formulations of Jatyadithailam in healing diabetic foot ulcers

Abstract

Jatyadithailam is a polyherbal formulation used by Ayurvedic practitioners and clinically reportedfor the treatment of inflammation related ailment specifically in non-healing chronic foot ulcers. The study is focused to validate and compare the Ayurvedic Formulary of India (AFI) and Yogagrantha (YG) formulations of Jatyadithailam in vitro, for its antimicrobial and anti-inflammatory potential. Antimicrobial activity of thailam was determined by broth microdilution method for its minimal inhibitory concentration (MIC) and microbicidal activity (MBC/MFC). In vitro anti-inflammatory activity of varying concentrations was determined by assaying albumin denaturation inhibition, membrane stabilization (hypotonicity-induced hemolysis), heat induced hemolysis and antiproteinase activities for both the formulations. Highest bactericidal and fungicidal activity was recorded by AFI formulation of Jatyadithailam showing low MIC values compared to YG formulation. Of all the tested bacterial strains, both the formulations showed great bactericidal effect against Staphylococcus aureus. Both the formulations exhibited significant dose dependent anti-denaturation and anti-proteinase effectively. The thailam also showed marked stabilization of human red blood cell (HRBC) membrane against thermal hemolysis. Our study confirmed AFI formulation of Jatyadithailam possessed better antimicrobial and significant (p<0.05) anti-inflammatory effect. AFI Jatyadithailam was more effective than YG formulation in terms of dose-dependence activity against infection causing microbes and toxic inflammatory mediators. The outcome of the study emphasizes the positive therapeutic potential of Jatyadithailam to combat infectious and inflammatory conditions.