Molecular Diversity Assessment in Selected Accessions of White Seeded Sesame (Sesamum indicum L.) Using SSR Markers

Abstract

Molecular characterization and genetic diversity among 50 sesame accessions was carried out by using 10 simple sequence repeat (SSR) markers. The study revealed enough genetic variability among the accessions at molecular levels. A total of 35 alleles with mean PIC of 0.42 obtained from the molecular analysis show the informative nature of SSR primers and their superiority in genetic diversity assessment. The bands produced by these primers considerably varied in size from 200 to 400 bp. The observed number of alleles per locus in all sesame accessions ranged from 3 to 6. The observed heterozygosity per primer ranged from 0.00 to 0.40 indicating a high degree of variation. The pair wise genetic similarity among 50 sesame accessions varied from 0.44 to 0.86. The dendrogram constructed based on genetic similarities among the accessions identified two major clusters, indicating high genetic resemblance among sesame accessions.