Diimide activated coupling of carbon nanotubes with chemically addressable peptide template for biomedical applications.

Abstract

The present work describes the preparation and application of functionalized carbon nanotubes (CNT) attached with biologically active peptide template. Multi walled carbon nanotubes (MWNT) is synthesized with stainless steel as a substrate without the use of any external catalyst by thermal chemical vapor deposition (thermal-CVD) method. The process is carried out in a cost effective way using a specially designed fabricated CVD furnace. A promising decapeptide template with free side-chain amino groups is synthesized on the PEGA support using solid phase peptide synthesis (SPPS). The sensitive biological element like cell receptors, enzymes, antibodies or nucleic acids can be attached to the side chains of this template. This deca peptide can be covalently attached to carbon nanotubes. The peptide-carbon nanotube scaffold synthesized can further be modified to detect signatures of cancer, disease-causing infectious agents and other pathological conditions.