Evaluation of bioavailability of three extracts of a less known ethno-medicinal plant, Thottea tomentosa from Assam

Abstract

Plants of the genus Thottea have been established as wild ethno-medicinal plants. The plant, Thottea tomentosa also has the illness recuperative property. However there are no such reports so far on the solvent polarity base performances of the plant.  Hence, the current work deals with the assessment of bioavailabilities like qualitative phyto-chemical screening, antioxidant efficiency, total phenol contents, total flavonoid contents and antimicrobial activity using various solvents of diverse polarities like hexane, chloroform, and methanol. Qualitative phyto-chemical screening exposes the stem part contains more phyto-chemicals than that of the leaf. In addition, phenolic compositions of methanol extract of stem and leaf; 374.99 ± 3.84 and 260.55 ± 4.00 mg GAE /g extract respectively comprises with a correlation between antioxidant activities of methanol extract of the stem and leaf (DPPH; IC₅₀ 202.39 ± 0.92 and 254.37 ± 1.47 µg/ml respectively). Thus, probable antimicrobial activities of methanol extract of stem and leaf were also study, than that of other extracts. Methanol stem extract against the microbes; Bacillus subtilis (MTCC 1427), Escherichia coli (MTCC 1195), Pseudomonas aeruginosa (MTCC 1688), Staphylococcus aureus susp.aureus (MTCC 1430), Streptococcus pneumonie (MTCC 2672),  Aspergillus niger (Lab isolates) and Candida albicans (MTCC 4748) exhibited highest zone of inhabitation (ZOI); 17.66 ± 1.45, 15.66 ± 0.88, 18.66 ± 0.66, 17.66 ± 1.45  19.33 ± 0.66 mm, 15.00 ± 1.15 and 14.66 ± 1.20 mm, respectively at concentration 20 mg/ml. Therefore, these results indicate that T. tomentosa can be satisfying the dietetic demand for the food and drug production.